Capillary electrophoresis is essential for microsatellite marker based detection and quantification of adulteration of basmati rice

Journal of Experimental Food Chemistry

ISSN: 2472-0542

Open Access

Capillary electrophoresis is essential for microsatellite marker based detection and quantification of adulteration of basmati rice

10th Global Summit on Food Safety, Processing & Technology

December 05-07, 2016 San Antonio, USA

Lakshmi Narayana R Vemireddy

Acharya NG Ranga Agricultural University, India

Posters & Accepted Abstracts: J Exp Food Chem

Abstract :

Rice is a staple and widely grown crop endowed with rich genetic diversity. As it is difficult to differentiate seeds of various rice varieties based on visual observation accurately, the harvested seeds and subsequent processed products are highly prone to adulteration with look-alike and low quality seeds by the dishonest traders. To protect the interests of importing countries and consumers, several methods have been employed over the last few decades for unambiguous discrimination of cultivars, accurate quantification of the adulterants, and for determination of cultivated geographical area. With recent advances in biotechnology, DNA based techniques evolved rapidly and proved successful over conventional non-DNA based methods to purge the problem of adulteration at commercial level. Microsatellite markers are employed for genotyping of basmati varieties and assaying purity of market samples. However, employment of diverse electrophoresis techniques across laboratories has resulted in inconsistent allele sizes, creating doubts about the suitability of the assay. This study evaluated agarose gel electrophoresis, slab gel electrophoresis, and capillary electrophoresis techniques for their efficiency in the detection and quantification of adulteration in basmati samples. Comparative analysis across eight microsatellite loci in 12 rice varieties demonstrated that the capillary electrophoresis method showed less error (0.73 bp) in the estimation of allele sizes compared to slab gel (1.59 bp) and agarose gel (8.03 bp) electrophoretic methods. Capillary electrophoresis showed greater reproducibility (<0.5 bp deviation) compared to slab gel (1bp) and agarose (>3 bp) based methods. Capillary electrophoresis was significantly superior in quantification of the adulterant, with a mean error of 3.91% in comparison to slab gel (6.09%). Lack of accuracy and consistency of the slab gel and agarose electrophoretic methods warrants the employment of capillary electrophoresis for Basmati rice purity assays.

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