Journal of Experimental Food Chemistry

César Lázaro de la Torre

Publications

• Research Article
  Development of HPLC-Fluorescence Method for the Determination of Ivermectin Residues in Commercial Milk
  Author(s): Saulo de Tarso Zacarias Machado, Aline Rodrigues Rezende, Solange Gennari, Carlos Adam Conte-Junior, Marion Costa, César Lázaro de la Torre and Evelise Oliveira TellesSaulo de Tarso Zacarias Machado, Aline Rodrigues Rezende, Solange Gennari, Carlos Adam Conte-Junior, Marion Costa, César Lázaro de la Torre and Evelise Oliveira Telles
  
  Objective: A study aimed at the validation of a high performance liquid chromatography (HPLC) method for detection and quantification of ivermectin (IVM) in commercial milk. Method: After a liquid-liquid extraction with acetonitrile and hexane, a derivatization with a mixture of trifluoroacetic acid and trifluoroacetic anhydride was performed. Chromatography conditions were a C8 column, a fluorescent detector (Ex. 360; Em. 470 nm), a run time of 25 minutes and an isocratic elution at 30°C. The methodology was validated in terms of selectivity, linearity, precision, recovery, limit of quantification (LoQ), limit of detection (LoD), and robustness. Results: The method showed an adequate linearity and selectivity (r2=0.999) with an elution time of 13.909 minutes. However, the repeatability and intermediate precision showed RSD values above of 20% and recovery between 37 to 79%. The c.. Read More»
  DOI: 10.4172/2472-0542.1000107

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