Department of Basic Sciences, University of Horticulture and Forestry, Himachal Pradesh, India
Research Article
Synthesis and Characterization of Gold Nanoparticle Conjugated Recombinant Aprv2 of Dichelobactor nodosus and its Immunogenicity
Author(s): Subhash Verma*, Geetanjali Singh, Mandeep Sharma, Pratiksha Srivastava, Richa Salwan and Chayan Nandi
The present study involves the synthesis, characterization and immunogenicity of Gold Nanoparticle (GNP) conjugated recombinant AprV2
protein of Dichelobactor nodosus. To achieve this, GNPs of size 20 nm were prepared and characterized. The gold nanoparticles
effectively adsorbed 3.3 μg of recombinant AprV2 protein over the surface. TEM studies revealed corona of AprV2 over GNPs (1-2
nm thick) in GNPs-AprV2 conjugate. Further, GNP-AprV2 conjugate revealed a red shift from 523 nm to 532 nm, indicating increased size of
GNPs after conjugation with AprV2. To evaluate the immunogenic response, mice were allotted to 5 different groups. Mice in group I injected
with GNP-AprV2 conjugate, group II with GNP-AprV2 with MPLA (Monophosphoryl lipid A), group III with AprV2 on its own, group IV with GNPs
and group V with Phosphate Buffered Saline (PBS). The highest ant.. Read More»
DOI:
10.37421/2476-1966.2023.8.186
Journal of Immunobiology received 34 citations as per Google Scholar report
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