Journal of Antimicrobial Agents

Mangum LC

Publications

• Research Article
  A Rapid, High-Throughput Iodometric Titration Method for the Determination of Active Chlorine Content of Topical Antiseptic Solutions
  Author(s): Mangum LC, Garcia GR, Niece KL, Wenke JC and Akers KSMangum LC, Garcia GR, Niece KL, Wenke JC and Akers KS
  
  Objective: A considerable number of commercially available topical antiseptic solutions rely on free available chlorine as an active ingredient due to its broad-spectrum antimicrobial activity, but limited empirical knowledge exists of the degradation kinetics of chlorine-based solutions in contact with host tissue. To better inform clinical practice, we developed and qualified a rapid and sensitive semi-automated microtiter plate-based iodometric titration assay suitable for the rapid determination of free available chlorine in small samples of dilute antiseptic solutions following contact with biological materials. Methods: The chlorine determination method described here utilizes a novel stepwise iodometric titration approach performed entirely on a plate reader spectrophotometer equipped with a standard automatic syringe dispenser module. I.. Read More»
  DOI: 10.4172/2472-1212.1000152

  Abstract PDF