Sahar Zafar, Saba Hanif and Rani Faryal
Quaid-i-Azam University, Pakistan
Scientific Tracks Abstracts: J Antimicrob Agents
The prevalence of kidney stones is a worldwide problem and also in Pakistan of 40-50% renal workload which is increasing rapidly. One of the threatening outcomes is nephrolithiasis with complicated Urinary Tract Infection (UTI) due to obstruction of stones within urinary tract. Klebseilla pneumoniae is reported as second most prevailing microorganism after E. coli to cause UTI among stone patients. The prevalence of K. pneumoniae among patients having kidney stone and kidney stone plus UTI was determined in tertiary care hospital of Islamabad. Among 121 patients, 71 were diagnosed with UTI. A total of 61 K. pneumoniae were isolated from 53 urine samples of UTI patients (63%) through morphochemical characterization. Out of these isolates, 18 were found to be hypervirulent strains of K. pneumoniae, these isolates were prevalent among kidney stone with UTI patients. These isolates had biofilm formation ability and resistance to 40% serum, in addition to hemolysin and hemagglutination in few isolates. Biofilm formation (P<0.015 and P<0.017) and serum resistance (P<0.008) and (P<0.005) was found to be strongly associated with BMI and age (31-50 years) of patients. One third of these isolates were resistant to more than one class of antibiotics, cephalosporins (65-72%) and aminoglycosides (37-49%), hence were termed as multidrug resistant. Much higher resistance (98%) was found for amoxicillin, clindamycin and TMP/SMX. Imipenem and nitrofurantoin were found to be most effective drug. Strong correlation was found between antibiotic resistance pattern of K. pneumoniae and its biofilm forming ability against ciprofloxacin (P>0.016), tobramycin (P>0.05) and amoxicillin (P>0.0005). Overall, K. pneumoniae isolates which were virulent and antibiotic resistant were found to be prevalent in kidney stone patients with UTI was seen suggesting that there might be role of K. pneumoniae in development of UTI among kidney stone patients.
Sahar Zafar is Ph.D. scholar in department of Microbiology, Faculty of Biological Sciences in Quaid-i-Azam University Islamabad Pakistan. She has completed her M.phil from Govt. College University Lahore. She has also done internship at National health Research Council Pakistan. She has published a paper of her M.Phil research in a reputed journal.
E-mail: [email protected]
Journal of Antimicrobial Agents received 376 citations as per Google Scholar report