NOD2/CARD15 gene polymorphisms are associated with increased risk of extensive chronic and steroidresistant graft versus host disease after hematopoietic stem cell transplantation

4^th Global Surgery and Transplantation Congress

October 03-04, 2016 Atlanta, USA

Victoria Lavrinenko, Kira Takun, Nina Minakovskaya, Dmitriy Prudnikov and Olga Aleinikova

National Research Center for Pediatric Oncology, Hematology and Immunology, Belarus

Posters & Accepted Abstracts: J Transplant Technol Res

Abstract :

GvHD (graft versus host disease) is a major cause of non-relapse mortality and morbidity after allogenic hematopoietic stem cell transplantation (HSCT). Polymorphisms of non-HLA genes including NOD2/CARD15 that influence on immune responses and inflamation may play a role in GvHD development. The aim of this study was to evaluate the influence of NOD2/CARD15 gene polymorphisms on the outcome of HSCT. 80 patients at the age of 0.5-29 (median, 9) years with malignant and non-malignant diseases after allogenic HSCT were included in the study. SNP8, SNP12 and SNP13 alleles of NOD2 had a frequency of 3.5%, 2.5% and 7.5%, respectively. Among donor/recipient pairs, no SNPs were detected in 64%, SNPs only in the recipient were in 9%, SNPs only in the donor were in 15%, SNPs both in the donor and the recipient were in 12%. The presense or absence of NOD2 polymorphisms in the donor and/or the recipient had no impact on OS, TRM, relapse incidence and acute GvHD. Extensive chronic GvHD was associated with the presence of NOD2 polymorphisms only in the donor compared to the group of patients without SNPs (50% vs. 15.8%, p=0.009). In the groups with SNPs only in the recipient or with SNPs both in the donor and the recipient, CI of extensive cGvHD was 13.3% and 14.3%, respectively. CI of steroid-resistant form was higher in the group with NOD2 SNPs only in the donor compared to the group of patients without polymorphisms (50% vs. 17.1%, p=0.009). In groups with SNPs only in recipient or with SNPs both in the donor and the recipient were 25% and 14.3%, respectively. SNPs of the NOD2 gene in donor and/or recipient are associated with a higher rate of infections during the first year after HSCT (93.1% vs. 72.6%, p=0.040). In conclusion, we found the evidence that NOD2/CARD15 polymorphisms in the donor influence on chronic and steroid-resistance GvHD and NOD2/CARD15 typing might help in donor selection.