Molecular Biology: Open Access

ISSN: 2168-9547

Open Access

Sabine Debuiche

ANSES, Laboratory for Food Safety, Bacteriology and Parasitology of fishery and aquaculture products Unit, Boulogne-sur-Mer, France

  • Research Article   
    Evaluation of Bacterial DNA Extraction Methods on Marine Samples Integrating a Process Control
    Author(s): Erwan Bourdonnais, Thomas Brauge, Sabine Debuiche, Cédric Le Bris and Graziella Midelet*

    To investigate the microbial community in the marine environment by molecular approaches, it is important to extract DNA in sufficient quantity and purity. The presence of inhibitors in the samples can lead to false negative results or a loss of information, but can be highlighted by a process control in the experiments. We compared seven bacterial DNA extraction methods on marine samples: fish skin, gills and guts, mollusk meat, phytoplankton and zooplankton. A process control (Listeria monocytogenes) was added in half of the samples. The performance of the DNA extraction methods were compared to produce the more pure and concentrated DNA for qPCR amplification targeting the bacterial tufgene and the process control hlyAgene. The purity and concentration of DNA were determined by spectrophotometry assays. The results showed that the .. Read More»
    DOI: 10.37421/2168-9547.2022.11.346

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