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Molecular Biology: Open Access

ISSN: 2168-9547

Open Access

Methods in Molecular Biology

The methods of molecular biology includes: 1) Hemacytometer for cell count, 2) Restriction enzyme digest(a process of cutting DNA molecules into smaller pieces with special enzymes called restriction endonucleases), 3) DNA ligation(using DNA ligase enzyme that helps in joining the DNA strands together by catalysing the formation of a phosphodiester bond), 4) Transfection(a process of introducing nucleic acids into the cells and used mainly for non-viral methods in eukaryotic cells), 5) Western Blot(also called protein immunoblot used as an analytical thechnique to detect specific proteins ina sample of tissue homogenate or extract and uses gel electrophoresis in separation of native proteins by 3-D structure or denatured proteins by length of the polypeptide), 6) Plasmid Purification(mainly used to isolate plasmid DNA from bacterial cells using various reagents like isopropanol chloroform etc., 7) ELISA(a test that uses antibodies and color change to identify a substance), 8) Electroporation, 9) Heat shock method, SDS-PAGE, DNAgel Electrophoresis, PCR, Passaging cells (which mainly expand the number of cells or microorganisms in the culture) also called subculturing and molecular cloning.

Related Journal of Methods in Molecular Biology

Molecular and Genetic Medicine, Journal of Steroid Biochemistry and Molecular Biology, International Review of Cell and Molecular Biology, Progress in Biophysics and Molecular Biology, Insect Biochemistry and Molecular Biology, Methods In Molecular Biology, Critical Reviews in Biochemistry and Molecular Biology.

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