Spain
Research Article
Application of Analyte Harvesting Nanoparticle Technology to the Measurement of Urinary HGH in Healthy Individuals
Author(s): Alessandra Luchini, Davide Tamburro, Ruben Magni, Claudia Fredolini, Virginia Espina, Jaume Bosch, Enrico Garaci, Emanuel F Petricoin III and Lance A LiottaAlessandra Luchini, Davide Tamburro, Ruben Magni, Claudia Fredolini, Virginia Espina, Jaume Bosch, Enrico Garaci, Emanuel F Petricoin III and Lance A Liotta
Urine represents a valuable biofluid for noninvasive measurement of Human Growth Hormone (HGH) secretion. Unfortunately, currently available commercial HGH immunoassays do not achieve the sensitivity needed for urinary HGH measurement in the low picogram per milliliter range, the expected normal concentration range of HGH in urine. A nanotechnology based sample preprocessing step was used to extract and concentrate HGH in urine so that urinary HGH could be measured with a clinical grade standard immunoassay designed for serum (Immulite 1000 ,Siemens). We applied the nanoparticle enhanced immunoassay to evaluate the baseline value of urinary HGH in a population of healthy young adults (age 18-30, N=33, median 21, M: F=39%:61%, with no reported medical therapies). Nanoparticle sample preprocessing effectively improved the lower limit of detection of the Immulite HGH assay by.. Read More»
DOI:
10.4172/2161-0673.1000116
Journal of Sports Medicine & Doping Studies received 1022 citations as per Google Scholar report