India
Research Article
Assessment of Immunogenic Potential and Protective Efficacy of Recombinant Whole Envelope and Domain III Subunit Vaccine Candidate against West Nile Virus
Author(s): Jyoti S Kumar, Divyasha Saxena and Manmohan ParidaJyoti S Kumar, Divyasha Saxena and Manmohan Parida
West Nile virus is an emerging arthropod borne flavivirus with increasing distribution worldwide that is responsible for severe and fatal encephalitis in humans. Currently, there is no licensed human vaccine or specific therapy to prevent WNV infection. Present study was aimed to assess the immunogenicity and protective efficacy of recombinant whole envelope (rEnv) and domain III (rDIII) in mice model. Briefly, the gene coding for the envelope and domain III protein of WNV was cloned and expressed in pET 28a expression vector followed by purification. Balb/C mice were immunized with the purified recombinant proteins of rEnv and rDIII with a conc. of 25 and 50μg with Freund' s adjuvant. Booster injections with same formulation were given on 14 and 28 days after first immunization. Two booster doses were given subsequently. Humoral and cell mediated immune responses were det.. Read More»
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