Research Centre on Chemical Processes Engineering and Forest Products (CIEPQF), Faculty of Pharmacy,
Coimbra
Portugal
Research Article
Topoisomerase I Improve JC virus DNA detection
Author(s): Ana Matos, Vitor Duque and Cristina LuxoAna Matos, Vitor Duque and Cristina Luxo
Introduction: Progressive Multifocal Leukoencephalopathy (PML) is a severe and often fatal CNS disease caused by JC human polyomavirus infection. Diagnosis of PML is based upon suggestive clinical symptoms and brain images, supported by the presence of JC virus genome in CSF samples.
Objective: The main objective of our study was the search for an alternative method for JC virus DNA detection in CSF samples, with sensitivity and specificity characteristics close to that of standard techniques, but feasible at any clinical laboratory.
Methods: In order to evaluate the effect of topoisomerase I treatment in the detection of JC virus genome, and its feasibility in laboratory diagnosis of PML, 129 CSF samples were examined for the presence of JC virus DNA by a nested-PCR protocol, with and without previous treatment with t.. Read More»
DOI:
10.4172/2168-9547.1000155
Molecular Biology: Open Access received 607 citations as per Google Scholar report
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