Journal of Antimicrobial Agents

T. arborvitae has been employed for its medicinal efficacy, especially for the treatment of bacterial infections. In this study, the aim was to isolate and analyze the antibacterial activity of bioactive materials from Thuja leaf extract. Both Gram-positive Staphylococcus aureus and Streptococcus mutans, as well as Gram-negative Acinetobacter baumannii, Escherichia coli, and Pseudomonas aeruginosa, were inhibited by the methanol extract. During the purification process, three distinct bioactive compounds P1, P2, P3 and P4 were separated. The purified compounds exhibited antibacterial activity with MICs between 10-50 µg/ml. All P1, P2, and P3 were remarkable against the multi-drug resistant strain Acinetobacter baumannii. These results support the effectiveness of the compounds from T. arborvitae proving their value in developing new therapeutic agents.