The response of HMSC-bm, SaOS-2 and immuno competent cells to Calcium Phosphate doped alginate coatings on Titanium surfaces

4^th International Conference and Exhibition on Materials Science & Engineering

September 14-16, 2015 Orlando, USA

Lucie Himmlova1, Dana Kubies2, Jirina Bartova1, Marta Vandrovcova3 and Elena Filova3

1Charles University, Czech Republic 2Institute of Macromolecular Chemistry, Czech Republic 3Institute of Physiology, Czech Republic

ScientificTracks Abstracts-Workshop: J Material Sci Eng

Abstract :

The release of calcium and phosphate ions into interface around orthopedic and dental implants may improve the bone healing. Human mesenchymal stem cells derived from the bone marrow (HMSC-bm), mononuclear cells obtained from buffy coat and connective tissue cell lines (SaOS-2) were used for testing of alginate hydrogel coatings on titanium grade 2, which were doped with in organic hydroxyapatite (Ti/ALG/HAP)or �²-tricalcium phosphate (Ti/ALG/TCP) nanoparticles. Uncoated titanium (Ti) and pure alginate (Ti/ALG) served as control surfaces. Samples were cultivated for 3 and7 days and cytokine production was assessed by multiplex proteomic analysis Ray Bio Human Inflammation Array (Ray Biotech, USA). Each cell type produces a different spectrum of cytokines. Mononuclear cells produce the most frequently factors of nonspecific immunity (MIP, RANTES, MCP-1, IL-6 andIL-8) in a dose higher than for the positive control. The cytokine production declined in the order Ti/ALG/HAPâ��Tiâ��Ti/ALG/TCPâ��Ti/ ALG. HMSC-bm produced mostly chemokines activating predominantly chemotaxis and activating monocytes, granulocytes and neutrophils, but in doses lower than mononuclear cells. SaOS-2 cells produced the broadest spectrum of cytokines but in low doses and with no significant difference between Ti/ALG/HAP and Ti/ALG/TCP surfaces. The immune response of mononuclear and stem cells showed differences between materials, whereas SaOS-2 cells werenâ��t sufficiently sensitive. Therefore, besides SaOS-2 cells, HMSC-bm and mononuclear cells should be also considered for in vitro evaluation of overall inflammatory response induced by presence of the implant.

Biography :

Lucie Himmlova has completed her PhD at First Faculty of Medicine, Charles University, Prague. She is teacher and research fellow at School of Dentistry and she was principal investigator of 6 projects. Her specialization is the surface treatment of implant and dental biomechanics. She has published more than 40 papers in international journals: This work was supported by IGA MH CR, project No. 13297-4 and by the European Regional Development Fund (project BIOCEV No. CZ.1.05/1.1.00/02.0109).

Email: himmlova@seznam.cz