Accepted Abstracts: J Material Sci
Growing evidence shows that physical microenvironments and mechanical stresses, independent of soluble factors, help influence mesenchymal-stem-cell fate. Here we demonstrate that modeled microgravity (MMG) can regulate the differentiation of mesenchymal stem cells into different directions, which might be a new strategy for tissue engineering and regenerative medicine. rMSCs were cultured respectively in normal gravity and in a clinostat to model microgravity for 72 h or 10 d, followed with diverse differential medium. The short time stimulation (72 h) promoted MSCs to endothelial, neuronal and adipogenic differentiation. On the contrary, the long time microgravity (10 d) promoted MSCs to osteoblast differentiation. Meanwhile, the short time intervention of MMG decreased RhoA activity significantly, but when we prolonged microgravity effect time to 10 days, the activity had a notable increase. When we used RhoA activity blocker, we explored that long time microgravity effects were reversed. We conclude that the duration of MMG could regulate the differentiation fate of mesenchymal stem cells via RhoA-related pathway.
Jun Chen has completed his Ph.D. at the age of 28 years from The Forth Military Medical University. He is the clinical doctor of department of encephalopathy in Traditional Chinese Medicine Hospital of Shan Xi Province. He has published more than 7 papers in reputed journals.
Journal of Material Sciences & Engineering received 3043 citations as per Google Scholar report