Ananda Kumar C S, Siddabasave Gowda B, Keiko Yamane, Okamoto Saori, Atsufumi Nakahashi, Mostafa A S Hammam, Tohru Taniguchi and Kenji Monde
Visvesvaraya Technological University, India
Frontier Research Center for Post-Genome Science and Technology, Japan
Hokkaido University, Japan
Posters & Accepted Abstracts: J Material Sci Eng
Sphingolipids comprise a complex range of lipids in which fatty acids are linked via amide bonds to a long-chain base or sphingoidbase backbone. Sphingolipids exist mostly in membranes and involved in a number of cell signaling systems. Sphingosines are a related class of long chain aliphatic compounds possessing two chiral centers which produce four stereoisomers and they are the backbone of more complex sphingolipids, including ceramide, sphingomyelin, cerebrosides and glycosphingolipids. These compounds are expressed on the surface of cell membranes and play an important role in cell-cell interactions, in signal transduction and in anchoring of proteins and also sphingosines are the inhibitors of protein kinase C. Due to their stereochemical interest and biological importance, the analysis methods for sphingosine and sphingolipids are therefore extremely important in drug discovery and as well is in life science. However conventional extraction methods of sphingolipids have been complicated with multistep protocols and no efficient stereochemical analysis method for biological samples has been established. Herein, we presenting an efficient extraction methodology by introducing a new concept called selective capture with catch and release process by chemoselective reaction utilizing glutaraldehyde resin and stereochemical analysis method using chiral HPLC technique.
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