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Polyacrylamide Gel | Open Access Journals
Chemical Sciences Journal

Chemical Sciences Journal

ISSN: 2150-3494

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Polyacrylamide Gel

Precast gels are able to use and offer greater convenience, more stringent internal control , and better reproducibility than handcast gels. Many precast gels also provide a time period of up to 12 months, allowing gels to be stored and used as required (this isn't possible with handcast gels, as they degrade within a couple of days).

Handcast gels, on the opposite hand, must be prepared from acrylamide and bis-acrylamide monomer solution; the component solutions are prepared, mixed together, then poured between two glass plates to polymerize (see Handcasting Polyacrylamide Gels for detailed protocols). Acrylamide and bis-acrylamide are neurotoxins when in solution, so care must be taken to avoid direct contact with the solutions and to wash up any spills. additionally , the casting process requires hours to finish and isn't as controlled because it is by gel manufacturers, so handcast gels are more irregular and fewer reproducible.

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