Rosmarinic acid, major polyphenol from Lemon balm (Melissa officinalis, L.) restrains non-enzymatic glycation of albumin

2^nd World Chemistry Conference

August 08-10, 2016 Toronto, Canada

Mehran Miroliaei

University of Isfahan, Iran

Scientific Tracks Abstracts: Chem Sci J

Abstract :

Rosmarinic acid (RA) is a major component of medicinal plants such as Melissa officinalis. The present study demonstrates the protection of human serum albumin (HSA) from fructation at autoxidative and glycoxidation stage by Rosmarinic acid. Attenuation of Millard reaction measured by absorbance at the early stage of glycation, provided evidence for RA preventive role on HSA fructation. CD spectropolarimetry demonstrated that the transition of �± to �² conformer at the glycoxidation stage can be overcome by addition of RA. Moreover, fluorescent monitoring of advances glycation endproducts (AGE) formation at emission wavelength of 440 nm revealed that RA blocks the late stage of fructation. Amyloid conformers were found to be progressively generated during fructation as mesured by ThT fluorescence. Suppresion of conformational alterations induced by ROS production suggested that RA inhibits the formation of cross �²-structures by modifying the transition of secondary and tertiary structures. The mechanism of RA action can be explained by the transistion metal ion chelating effect of RA in the oxidative reactions of AGE formation and/or by RA influence on the recations of dicarbonyl intermediates of AGE formation. The results indicate that the multi-target mode of RA action have promising potential for prevention of diabetic complications arising from HSA glyctation.

Biography :

Mehran Miroliaei is currently working as an Associate Professor Department of Biology, Faculty of Sciences, University of Isfahan, Iran.
Email:mmiroliaei@yahoo.com