Catalysis Importance

Millions of protein sequences are being come across at an incredible pace, representing an inexhaustible source of biocatalysts. Despite genomic databases growing exponentially, classical biochemical characterization techniques are time-demanding, cost-ineffective, and low-throughput. Therefore, computational methods are being developed to explore the unmapped sequence space efficiently. The selection of putative enzymes for biochemical characterization based on rational and robust analysis of all available sequences remains an unsolved problem. To address this challenge, we have come about EnzymeMiner—a web server for automated screening and annotation of diverse family members that enables the selection of hits for wet-lab experiments. EnzymeMiner prioritizes sequences that are more likely to preserve the catalytic activity and are heterologously expressible in a soluble form in Escherichia coli. The solubility prediction employs the in-house SoluProt predictor developed using machine learning. EnzymeMiner reduces the time devoted to data gathering, multi-step analysis, sequence prioritization, and selection from days to hours. The successful use case for the haloalkane dehalogenase family is described in a comprehensive tutorial available on the EnzymeMiner web page.

High Impact List of Articles

Conference Proceedings

Phenylalanine/Tyrosine ammonia lyase enzyme catalyzed synthesis of the methyl ester of parahydroxycinnamic acid and its potential antibacterial activity
Godwin Basil Dâ�?�?Cunha
Scientific Tracks Abstracts: Chemical Sciences Journal
Phenylalanine/Tyrosine ammonia lyase enzyme catalyzed synthesis of the methyl ester of parahydroxycinnamic acid and its potential antibacterial activity
Godwin Basil Dâ�?�?Cunha
Scientific Tracks Abstracts: Chemical Sciences Journal
Supramolecular catalysis within confined environment of metal-organic architectures
Chunying Duan
Scientific Tracks Abstracts: Chemical Sciences Journal
Supramolecular catalysis within confined environment of metal-organic architectures
Chunying Duan
Scientific Tracks Abstracts: Chemical Sciences Journal
3-Nitro-2-(trihalomethyl)-2H-chromenes in Reactions with 1,3-Dipoles: Synthesis and Cytotoxic Activity of â�?�?3-Annulated Chromane Derivatives
Vladislav Yu Korotaev, Nikolay S Zimnitskiy, Igor B Kutyashev, Alexey Yu Barkov and Vyacheslav Ya Sosnovskikh
Scientific Tracks Abstracts: Medicinal Chemistry
3-Nitro-2-(trihalomethyl)-2H-chromenes in Reactions with 1,3-Dipoles: Synthesis and Cytotoxic Activity of â�?�?3-Annulated Chromane Derivatives
Vladislav Yu Korotaev, Nikolay S Zimnitskiy, Igor B Kutyashev, Alexey Yu Barkov and Vyacheslav Ya Sosnovskikh
Scientific Tracks Abstracts: Medicinal Chemistry
Neuroprotective effect of tectoridin in SH-SY5Y cells with oxidative stress and transient global ischemia model in gerbil
Sung-Jin Lee, Hyun Ahm Sohn and Woongchon Mar
Accepted Abstracts: Medicinal Chemistry
Neuroprotective effect of tectoridin in SH-SY5Y cells with oxidative stress and transient global ischemia model in gerbil
Sung-Jin Lee, Hyun Ahm Sohn and Woongchon Mar
Accepted Abstracts: Medicinal Chemistry
What can we expect from new therapeutic strategies in nano-pharmacology and nanomedicine?
Hans-Christian Siebert
Scientific Tracks Abstracts: Medicinal Chemistry
What can we expect from new therapeutic strategies in nano-pharmacology and nanomedicine?
Hans-Christian Siebert
Scientific Tracks Abstracts: Medicinal Chemistry

Chemical Sciences Journal