Journal of Bioprocessing & Biotechniques

Department of Medicinal and Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India

Publications

• Research Article   
  Whole-Cell Biocatalysis for 4-Hydroxyisoleucine (4-HIL) production in E. coli
  Author(s): Gaurav Prajapati, Meenakshi Pawar and Kinshuk Raj Srivastava*
  
  4-HydroxyIsoLeucine (4-HIL) is a valuable non-proteinogenic amino acid with potent insulinotropic properties, offering significant therapeutic potential for the treatment of type 2 diabetes and metabolic disorders. Currently, the industrial availability of 4-HIL is limited by the low yields obtained from Trigonella foenum-graecum (fenugreek) seeds and the environmental burden associated with chemical synthesis. To address these limitations, this study establishes a sustainable whole-cell biocatalytic platform for the production of 4-HIL using recombinant Escherichia coli containing L-isoleucine dioxygenase (IDOI162T/T182N). Thus, a whole-cell biotransformation system was subsequently developed, wherein the engineered cells catalysed the regio- and stereoselective hydroxylation of exogenously supplied L-isoleucine in the presence of α-ketoglutarate, FeSO? and ascorbic acid. This .. Read More»
  

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