Journal of Antimicrobial Agents

Controlling of Halophilic Isolates Using Thiocyanomethylthiobenzothiazole, Potassium Dimethyldithiocarbamate, and benzyloxymethanol

Abstract

Baidaa M. Kadim* and Haider S. Abdulhusein

The hides and skins are contaminated by bacteria that are found in the atmosphere, soil, water, dung, and slaughterhouses. Prior investigations have discovered antibiotic-resistant gam-positive and gram-negative bacteria in both the liquids used for soaking and the hides that were soaked, despite the use of several antimicrobial agents during the soaking process. Optimal concentration of the antimicrobial agent is essential to ensure the soaking process is carried out efficiently and effectively. Hence, it is feasible to control bacterial contamination on the skin. This study aimed to investigate the Minimum Inhibitory Concentrations (MICs) of antimicrobial agents containing three different chemical materials: Thiocyanomethylthiobenzothiazole, potassium dimethyldithiocarbamate, and benzyloxymethanol. Each of these active ingredients was tested separately at different concentrations against gram-positive bacteria, including Staphylococcus haemolyticus, Staphylococcus taiwanensis, Staphylococcus aureus, Staphylococcus epidermidis, Peribacillusfrigoritolerans, Bacillus haynesii, Bacillus piscis, and Corynebacterium aurimucosum. Also against gram-negative bacteria Pseudomonas iranica and Pseudomonas oryzihabitans, those were previously obtained from leather industry. In addition, to assess the susceptibility of these isolates to eight different antibiotics: Cefotaxime (5 μg), Amikacin (30 μg), Spectinomycin (100 μg), Tetracycline (30 μg), Rifampicin (5 μg), Cephalothin (30 μg), Sulbactam/ Ampicillin (10 μg), and Neomicin (30 μg). The Kirby-Bauer disc diffusion susceptibility test method was employed on Mueller Hinton agar. In addition, this investigation utilized 22 different doses of antimicrobial chemicals that had three different active components. The concentrations ranged from 2500 μg/ml to 0.001 μg/ml for thiocyanomethylthiobenzothiazole, 5000 μg/ml to 0.002 μg/ml for potassium dimethyldithiocarbamate, and 10000 μg/ml to 0.0047 μg/ml for benzyloxymethanol as the active ingredient. The results found that the growth of all tested isolates was inhibited after exposure to the concentrations of antimicrobial agents containing thiocyanomethylthiobenzothiazole as an active ingredients started from 2500 μg/ml to 39.06 μg/ml, 5000 μg/ml to 156.25 μg/ml containing potassium dimethyldithiocarbamate, and 1000 μg/ml to 312 μg/ml containing benzyloxymethanol. This investigation will be useful if it shares with industries.