Transcriptional regulation of leucine-rich repeats and WD repeat domain containing 1 (LRWD1) by microRNA under reactive oxygen species

15^th Asia-Pacific Biotechnology Congress

July 20-22, 2017 Melbourne, Australia

Teng Yen-Ni, Chia-Hui Su, Ming-Syuan Wu, Jie-Yun Tseng and Hsing-Yi Chen

National University of Tainan, Taiwan

Posters & Accepted Abstracts: J Bioprocess Biotech

Abstract :

LRWD1 (Leucine-Rich repeats and WD repeat domain containing 1) is highly expressed in the testes and down-regulated in the testicular tissues of the patients with severe spermatogenic defects. In previous study, LRWD1 expressed in the centrosome of sperm and involved in microtubule growth. MicroRNA is an important factor of post-transcription regulation and regulates the translation and expression for gene. In the bioinformatics prediction, there are binding sites for miR-320a and miR-450a in the LRWD1 3�UTR by miRanda software and high expression at reproduction tissue for miR-320a and miR450a by miRnaMap software. The LRWD1 3�UTR was constructed to the pMIR plasmid and confirmed that miR-320a, not miR- 450a, increased LRWD1 expression by Dual-Luciferase Reporter Assay. LRWD1 expression increased by transfected miR-320a mimic, but not in miR-450a mimic in NT2D1 cells. The expression of miR-320a and LRWD1 were increased by hydrogen peroxide (H2O2) or Sodium Nitroprusside Dehydrate (SNP) treatment in TaqMan real time-PCR assay. Transfection mimic of miR-320a and miR-450a enhanced cell growth, but not in miR-450a. With this study, the post-transcriptional regulation of miR-320a and miR-450a for LRWD1 will help us understand the function and roles of the miRNA in post-transcriptional regulation of LRWD1 and may provide a rationale for further diagnosis and treatment of spermatogenic defect and male infertility diseases.

Biography :

Teng Yen-Ni has her expertise in human genetics and reproductive medicine. She used the molecular biology technology and genetics in gene detection and congenital disease analysis.

Email: tengyenni1968@gmail.com