Production, isolation, screening and extraction of polyhydroxybutyrate (PHB) from Pseudomonas sps. and E. coli using treated sewage sample

3^rd International conference on Bioprocess and Biosystems Engineering

September 14-15, 2015 Baltimore, USA

K Swathi

St. Francis College for Women, India

Posters-Accepted Abstracts: J Bioproces Biotechniq

Abstract :

Improper disposal of plastics has threatened natural environment worldwide. Conventional petrochemical plastics due to their excessive molecular size are resistant to biodegradation and are persistent in soil for long time. To overcome this problem, the production of eco-friendly products such as biodegradable plastics becomes inevitable. Polyhydroxybutyrate (PHB) which is produced by bacteria provides an alternative to petrochemical plastics. PHBs are polyesters that are accumulated as intracellular granules which are stored form of carbon and energy materials. PHBs are attracting considerable attention as biodegradable plastics due to their good barrier properties. These polymers exhibit thermoplastic and elastomeric features in addition to other interesting properties, such as biodegradability and biocompatibility, which make them promising industrial materials. The present study aims at the isolation and production of PHB from Pseudomonas sps. and E. coli. Treated sewage samples were collected form, Sewage Treatment Plant, Pragathi Nagar, Kukatpally, Hyderabad, for isolation of PHBs from Pseudomonas sps. and E. coli. PHB granules are identified by Sudan black B staining and standard curve of PHB is studied by double beam UV-Vis Spectrophotometer. Present investigation revealed the utilization of treated sewage sample for bioplastic production which draws solution for proper disposal of treated sewage water. The medium used for the PHB isolation and production was simple and cost effective. Information regarding these studies will be elucidated in the presentation.

Biography :

Email: kswathi1009@gmail.com