Process development for lab scale xylitol production and design of pilot scale stirred tank reactor

Journal of Bioprocessing & Biotechniques

ISSN: 2155-9821

Open Access

Process development for lab scale xylitol production and design of pilot scale stirred tank reactor

3rd International conference on Bioprocess and Biosystems Engineering

September 14-15, 2015 Baltimore, USA

R Balakrishnaraja

Bannari Amman Institute of Technology, India

Posters-Accepted Abstracts: J Bioproces Biotechniq

Abstract :

Combating the complex disorders such as Diabetes, Cardiac malfunctions and tumors will be possible by suitable regulatory pathways. Xylitol is a polyalcohol having relative sweetness equivalent to fructose and doesn├ó┬?┬?t involve in insulin metabolic pathway. It also acts against dental cavities as anticariogenic compound in pharmaceutical ingredients. The present study deals about the production of xylitol in a two liter stirred tank bioreactor and estimation of its oxygen mass transfer coefficient with corn hydrolysate medium. Then organism employed here was isolated & identified from cane processing industry through standard biotechnological techniques. Corncob was milled, pretreated, hydrolyzed and used as supplementary medium with 49% initial xylose content (w/v). Physiological conditions such as pH, temperature, inoculum volume and agitation speed was optimized using response surface methodology and adopted in bioreactor. The oxygen side mass transfer coefficient was also measured using dynamic gassing method. The stirred tank reactor was designed according to the standard procedures and all materials used for fabrication were stainless steel. The vessel has 17 liters working volume with aspect ratio for H:D as 4:1.

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