Nasrin Afsarimanesh, Subhas Mukhopadhyay and Marlena Kruger
Macquarie University, Australia
Massey University, New Zealand
Posters & Accepted Abstracts: J Biosens Bioelectron
This research proposes a novel real-time, label-free sensing technique for the detection of C-telopeptide of type-I collagen (CTx-I) that can be heelpful in early detection of bone loss. A planar interdigital sensor in conjunction with electrochemical impedance spectroscopy (EIS) was used to study the dielectric properties of the test sample. Molecular imprinted polymer (MIP), including artificial recognition sites for CTx-I molecules was synthesized by precipitation polymerization using CTx-I peptide as a template, ethylene glycol methacrylate as the cross-linker and methacrylic acid as a functional monomer. The sensor was coated using the prepared polymer in order to make the sensor selective for CTx-I molecule. Calibration experiments were performed using different known concentration samples and the reference curve was plotted. Complex non-linear least square (CNLS) curve fitting method was used to estimate electrochemical equivalent circuit parameters. The developed system showed a detection limit of 0.1 ng/ml. Two different unknown samples obtained from sheep blood were measured using the developed sensing system and enzyme-linked immunosorbent assay (ELISA) was used to validate the results.