Hamid Mukhtar, Umar Farooq Gohar and Ikram-ul-Haq
GC University, Pakistan
Posters-Accepted Abstracts: J Bioproces Biotechniq
This study was aimed at the isolation of the highly productive fungal strain for the production of an anti-cancer drug taxol. About 140 samples belonging to ten plant species were collected from different localities of Lahore, Nathia Gali and Shringal (Dir). About 300 fungal strains were isolated and screened for the production of the taxol using surface culture fermentation. Fifteen isolate were found to have the ability to produce the taxol. The maximum amount of the taxol, i.e., 745 ├?┬╝g/L of the culture broth was produced by the strain IIB 275. The said strain was identified as Eurotium rubrum on the basis of morphological features and DNA sequence analysis. Ten different fermentation media were optimized and M3 was found to be the best with maximum yield of the taxol (2.58 mg/L). The incubation temperature and period was also optimized and it was found that the fungal strain had maximum productivity at temperature 30├?┬░C (2.59 mg/L) and incubation period of 21 days (3 mg/L). The fermentation medium with initial pH of 6.5 was found to have the maximum productivity of the taxol. Among the nutritional parameters, glucose with concentration of 4% was found to be the best carbon source while peptone and sodium nitrate was found as best organic and inorganic nitrogen sources, respectively. A 5% spore inoculum was found to have the maximum productivity of the taxol (8.50 mg/L) at optimized fermentation conditions.