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Downstream process development for purification of a novel anti-MRSA antibody: A case study for antibody aggregation removal
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Journal of Bioprocessing & Biotechniques

ISSN: 2155-9821

Open Access

Downstream process development for purification of a novel anti-MRSA antibody: A case study for antibody aggregation removal


3rd International conference on Bioprocess and Biosystems Engineering

September 14-15, 2015 Baltimore, USA

Yanfeng Zhang

XBiotech, USA

Scientific Tracks Abstracts: J Bioproces Biotechniq

Abstract :

Staphylococcus aureus is gram-positive bacteria that commonly causes human health problem such as skin and respiratory infections, and food poisoning. S. aureus has multiple virulence factors including henolysins, toxins and superantigens, and the strains have high genetic variability. Some strains of S. aureus are also resistant to antibiotics (MRSA and VRSA), resulting in an infection that is difficult to control. XBiotech has screened the blood of hundreds of human individuals to find antibodies that directly and specifically target S. aureus. One of these true-human antibodies, 514G3, has shown positive results in preclinical studies against S. aureus and is currently being evaluated in a Phase I/II study. During the purification of 514G3 antibody, an unusually high level of protein aggregation (up to 20%) was observed. Therefore, the protein aggregation problem was first analyzed by a series of analytical methods. Then, downstream processes were developed to remove the aggregated antibodies. This presentation will describe our strategies of resin screen and optimization for the removal of antibody aggregates.

Biography :

Yanfeng Zhang completed his PhD from Michigan State University and Post-doctoral studies from Pacific Northwest National Laboratory and University of Texas Health Science Center at San Antonio. He is Senior Scientist of Process Development of XBiotech, USA, the world’s leading developer of next-generation True Human therapeutic antibodies. He has published more than 20 papers in reputed journals and has been serving as an editorial board member of international journals.

Email: yzhang@xbiotech.com

Google Scholar citation report
Citations: 3351

Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report

Journal of Bioprocessing & Biotechniques peer review process verified at publons

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