Detection of lamp amplified Amelogenin gene using a binary deoxyribozyme sensor

5^th International Conference on Forensic Research & Technology

October 31-November 02, 2016 San Francisco, USA

Alexandra Smith, Tosha Dupras and Dmitry Kolpashchikov

University of Central Florida, USA

Posters & Accepted Abstracts: J Forensic Res

Abstract :

Sex identification of unknown remains is crucial to personal identification of human remains in anthropology and forensics. When conventional methods, such as metric or morphological analysis, are not an option due to the fragmented or prepubescent remains, molecular diagnostics are needed. The amelogenin gene, found on sex (X and Y) chromosomes, is the most common molecular marker used for sex determination because it exhibits sexual dimorphism in size and sequence. Here we develop a new method for fluorescent and visual easy-to-use analysis of amelogenin gene for sex identification. In this assay, human DNA is amplified during a period of 15 min by isothermal loop mediated amplification (LAMP) followed by analysis by a binary deoxyribozyme sensors for 60-20 min. High selectivity of the amelogenin sequences of Y and X chromosome was demonstrated. The assay promises to simplify molecular-based sex determination of human remains.

Biography :

Email: alexandra.s@knights.ucf.edu