Characterization of lignin peroxidase from Paecilomyces species for decolorisation of pulp and paper mill effluent

3^rd International conference on Bioprocess and Biosystems Engineering

September 14-15, 2015 Baltimore, USA

Pratibha Singh

JSS Academy of Technical Education, India

Posters-Accepted Abstracts: J Bioproces Biotechniq

Abstract :

Eight fungal strains (F1 to F8) isolated from the sediment core and degraded wood of the pulp and paper mill effluent and Phanerochaete chrysosporium were tested for different cellulolytic (CMcase, FPase, Xylanases) and lignolytic (lignin peroxidase) enzyme activity in pulp. F-3 (Paecilomyces sp.) strain showed higher enzyme activity compare to other fungi isolated from effluent. Weight loss, cellulose loss and organic carbon contents were found maximum in F-3 (Paecilomyces sp.) strain. Reducing sugar, protein content and colour removing potential was also higher in Paecilomyces sp. The culture extract of F-3 strain grew in presence of pulp fractionated into five protein fractions had indicated two fractions of molecular weight, 38,000 and 40,000 Da, having lignin peroxidase activity. Lignin peroxidase had optimum activity at pH 2 to 3 and temperature 20-30 �°C, maximum activity at 6 mM to 48 mM veratryl alcohol concentration and 256 mM H2O2, however, sodium azide inhibits the enzyme activity. Different metals (CoCl2, HgSO4, CaCl2, SnCl2, FeSO4, CuSO4 and ZnSO4) also affected the lignin peroxidase activity.

Biography :

Email: Pratibhasingh@jssaten.ac.in