Characterization and biotransformation of racemic analogues of potent calcium channel blockers

2^nd World Chemistry Conference

August 08-10, 2016 Toronto, Canada

Jaspreet Kaur, Ranju Bansal and Anupreet Kaur

Panjab University, India

Posters & Accepted Abstracts: Chem Sci J

Abstract :

1, 4-dihydropyridines also popular as calcium channel blockers have been widely used in the clinical medicine as potent drug candidates in the treatment of vasodilation and other cardiac disorders. This class of drug possesses the plethora of biological activities as a result of different functional groups substitutions on the Dihydropyridine (DHP) moiety. Such molecules possessing asymmetric carbon atom at position 4 become chiral, and thus exist as two stereo-isomers. The activity of each enantiomer may differ, one being calcium antagonist of the channel and the other as agonist. In the light of the structural and chiral variations of DPH moiety, it becomes important to investigate and establish the structures of these isomers in order to prepare the title compounds in enantiomerically pure form. For this purpose, racemic mixtures of these title compounds were synthesized and structures were elucidated via NMR, IR and MS analysis. Normal phase liquid chromatographic characterizations of the racemic mixtures have been optimized with retention time below 10 minutes. Further, the transformation on the racemic mixtures has been investigated by subjecting them to enzymatic treatment at different concentrations and temperatures. It has been found that enzymatic treatment with hydrolases has led to change in the optical rotation values indicating stereo selective transformation. Further work is being done to resolve them into enantiomerically pure compounds with chiral HPLC. jaspreet_virdi@yahoo.com