Bioconversion of rubber seed to produce protein and oil rich-biomass employing black soldier fly larvae assisted by microbes

15^th Asia-Pacific Biotechnology Congress

July 20-22, 2017 Melbourne, Australia

Muhammad Yusuf Abduh

Bandung Institute of Technology of, Indonesia

Posters & Accepted Abstracts: J Bioprocess Biotech

Abstract :

A co-conversion process using black soldier fly larvae (Hermetia illucens) and microbes was studied to convert rubber seed into insect biomass containing high amount of protein and oil. De-oiled rubber seeds (cake to shell ratio of 2.5 wt.%) was mixed with water (water to biomass ratio of 1.5-2.3 wt.%) and pre-treated with two types of microbes; liquid culture of Aspergillus niger (107 spore/ ml) and biostaff solution containing consortium of microbes (107 cell/ml). Black soldier fly larvae were reared in rearing containers (6.8Ã?Â?6.8 cm) with a larval density of 5 larvae/cm2 and a feeding rate of 68 mg/larvae/d. The rearing containers were subjected with different shade rate (25-98%). After 12 days of treatment, the final average weight of the prepupae lies in the range of 7.2 to 19.5 g with an estimated biomass productivity of 133 to 358 g/m2.d. The efficiency of black soldier fly larvae in converting the digested feed is 12.5 to 25.9% with an assimilation efficiency of 22.9 to 28.7%. The waste reduction index is estimated at 2.1 to 2.6. The protein and oil content of the prepupae was analyzed to evaluate the potential of pre-treated rubber seed as a substrate for the cultivation of black soldier fly larvae to produce protein and oil-rich biomass. The prepupal biomass has a relatively high protein (28.6-55.2 wt.%, d.b.) and oil content (18.9 to 28.3 wt.%, d.) and is suitable for application as cattle feed.

Biography :

Email: yusuf@sith.itb.ac.id