Authentication and characterization of animal cell lines: Towards best practices in cell culture

3^rd International conference on Bioprocess and Biosystems Engineering

September 14-15, 2015 Baltimore, USA

Yvonne A Reid

American Type Culture Collection, USA

Posters-Accepted Abstracts: J Bioproces Biotechniq

Abstract :

Animal cell lines are important in vitro systems and tools for scientists in diverse disciplines such as basic cell biology, genetic mapping, gene expression and gene therapy. Cell line authentication and characterization are crucial in these activities. Yet, they are underappreciated by most research scientists. Over the years, numerous cell lines have been shown to be misidentified due, in part, to poor techniques and inadequate authentication protocols. Technological advances have given rise to improved capabilities. Cell line authentication and characterization now require a comprehensive strategy that employs several complementary technologies for systematic testing for morphology, microbial contaminations, cellular crosscontamination as well as functionality. The validity of conclusions drawn from research data is dependent on consistent and unequivocal verification of cell line identity and function. It is estimated that the financial loss incurred by poorly characterized or misidentified cell lines is in the millions of dollars. An overview of the current technologies used to authenticate and characterize animal cell lines will be presented.

Biography :

Email: yreid@atcc.org