A chemiluminescence approach for the detection of morphine in urine samples

Biosensors & Bioelectronics

ISSN: 2155-6210

Open Access

A chemiluminescence approach for the detection of morphine in urine samples

6th International Conference and Exhibition on Biosensors & Bioelectronics

September 22-23, 2016 Phoenix, USA

Sonu Gandhi

Amity University, India

Posters & Accepted Abstracts: J Biosens Bioelectron

Abstract :

A sensitive immunoassay for morphine was developed by chemiluminescent approach. The anti-MAM antibody was developed for Monoacetyl Morphine (MAM) and labeled with horseradish peroxidase (HRP) for the development of competitive assay. The method is based on the competition between labeled antibodies and free drug in spiked urine samples. A hapten (MAM) was functionalized with chloroacetic acid chemistry to generate hydroxyl moiety and conjugated with carrier protein (BSA). The biophysical characterization was done by various spectroscopic methods such as UV-Vis, fluorometer, CD, IR, MALDI-TOFF and was used as an immunogen for the generation of anti-MAM antibody. A binding assay was performed and high titer of antibody (1:64,0000) was obtained and the relative binding affinity constant (Kaff) of anti-MAM antibody was 3.1??107 l/mol. Under competitive conditions, the IC50 value for heroin, mono acetyl morphine (MAM), morphine and codeine were in the range of 0.01-0.08 ng/ml respectively. The developed chemiluminescence based competitive assay could detect heroin and its metabolites in standard and urine samples up to 0.01 ng/ml that indicates fairly good precision with linear range, reactivity and therefore could be implemented in illegal trafficking.

Biography :


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