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Adaptation To Serum | Open Access Journals
Journal of Blood & Lymph

Journal of Blood & Lymph

ISSN: 2165-7831

Open Access

Adaptation To Serum

Suspension cultures without serum are preferably required for the production of recombinant proteins due to its availability in upstream / downstream processing and scaling up, thereby increasing the productivity and competitiveness of the process. This type of culture replaces traditional cell culture because the presence of components of animal origin can introduce a lot and a lot of adventitious pathogens into the process. However, the adaptation of cells to serum-free conditions is difficult, time-consuming and depends on the cell line and the medium. In this chapter, we present different approaches that can be used to adapt mammalian cell lines from an anchor-dependent serum-supplemented culture to a suspension-free serum culture.

 

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Citations: 443

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