Journal of Pharmacognosy & Natural Products

ISSN: 2472-0992

Open Access

S.A. Sreenivas

Department of Quality assurance, Mewar University, Chittorgarh, Rajasthan, India

  • Research Article   
    New RP-HPLC Method Development and Validation for Simultaneous Estimation and Forced Degradation Studies of Gallicacid and Curcumin in Solid Dosage Form
    Author(s): S.K. Godasu* and S.A. Sreenivas

    Objective: A New method was established for simultaneous estimation of Gallic acid and curcumin by RP-HPLC method. Methods: Chromatographic separations were carried using Inspire (4.6 × 150 mm, 5 m) column with a mobile phase composition of 0.1% OPA buffer and Acetonitrile (30:70) have been delivered at a flow rate of 1 ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 with PDA detector 2996 at wavelength 260 nm. Results: The retention time for Gallic acid and curcumin were 2.119 and 2.730 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 500-2500 μg/ml for Gallic acid and 5-25 µg/ml for curcumin respectively. For accuracy the total recovery was found to be 100.58% and 100.54% for Gallic acid and curcumin. LOD and LOQ for gallic acid 3.05 a.. Read More»
    DOI: 10.37421/2472-0992.2022.8.191

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