Journal of Pharmacognosy & Natural Products

ISSN: 2472-0992

Open Access

Articles in press and Articles in process

    Research Article Pages: 1 - 9

    Phytochemical Influence ofSalvia officinalisL . from Saudi Arabia and Palestine on Oxidation and HIV Viral Load

    Maryam A. Aljaziri, Dina Hajjar, Abdul-Hamid Emwas, Arwa A. Makki*and Mariusz Jaremko

    Background: This study sought to identify and characterize the diverse flavonoids and phytochemical compounds in Saudi and Palestinian Salvia officinalis (sage) leaf extracts, and to evaluate their ability to inhibit protease enzymes, and thus their potential for use in antiretroviral drug discovery and development. We also explored sage’s ability to scavenge radicals; antioxidants derived from Lamiaceae plants play an important role in natural medicine.

    Methods: The crude extract of methanol and dichloromethane solvents from Saudi and Palestinian Salvia officinalis leaves (sage) were in vitro screened for inhibitory activity against HIV-1 protease. We examined antioxidant capacity against the synthetic radicals of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), identified the chemical constituents of the extracts by gas chromatography–mass spectrometry, ultrahigh-pressure liquid chromatography and nuclear magnetic resonance, and determined the diversity of flavonoids by liquid chromatography.

    Results: Regarding the antiviral results, both Saudi and Palestinian sage leaf extracts significantly inhibited HIV-1 PR activity when compared to the vehicle control which was approximately (25.1 ± 10.6) µg/mL. The investigation of antioxidant capability revealed significant scavenging of synthetic ABTS radicals by Palestinian Salvia officinalis (sage) leaf extracts compared to the Saudi sage leaf extracts. Chemical analyses revealed a wide variety of secondary and primary compounds and flavonoids which were compared.

    Conclusion: In this study we have investigated and revealed a variety of biological compounds that contribute to the inhibition of oxidative stress and protease enzyme. The results provide promising and beneficial data for the discovery and development of novel drugs against oxidation and HIV/AIDS.

      Research Article Pages: 1 - 14

      Comparison of Different Solvent and Extraction Methods for Isolation of Flavonoids Compound from Leaves of Clerodendrum Infortunatum Linn

      Abhijit N. Daf* , Shekhar B. Waikar, Akshay N. Madavi, Akash S. Kapse, Shailesh B. Pise, Dhananjay G. Sawarkar and Dharmendra Mundhada

      Clerodendrum infortunatum Linn leaves of the Verbinaceae Family and contains biologically active substances. The aim of the current research was to determine best methods for extraction in different solvents and evaluation of different extraction methods for best of flavonoid compounds. Clerodendrum infortunatum Linn leaves was extracted with four different solvents. Extraction of the plant material with various organic solvents in increasing order of polarity with the help of Petroleum ether (60-80°), Chloroform, Acetone and Methanol solvents to the find out the percentage (%) yield of all the extracts. Thin layer chromatography study of each extract to know the number of components present in them. Extraction of flavonoid rich fraction with the help of (80%) ethanol (N.R Fransworth) by using different extraction methods then comparative study of percentage yield of total flavonoids like Maceration, Hot Continuous Percolation (Soxhlet Extraction), Microwave assisted Extraction and Ultrasonic Extraction (Extraction using Ultrasonic waves) and collect the fraction using column chromatography and Identification of isolated compound with the help of UV spectroscopy either Quercetin, Rutin or any other suitable flavonoid marker.

        Commentary Pages: 1 - 1

        Essential Oils of Aromatic and Medicinal Plants as Botanical Biocide for Management of Coconut Eriophyid Mite (Aceria guerreronis Keifer)

        Hadiza Fadel

        The eriophyid parasite (Aceria guerreronis Keifer) is an infinitesimal creature that stays under the perianth of the coconut and has been one of the genuine vermin of coconut throughout the previous thirty years in significant coconut developing nations. These little bugs total in states in the internal and external bracts and under the tepals and feed on the meristematic tissues on the nut surface. Because of bug assembly and taking care of the meristematic tissue underneath the perianth becomes chlorotic and afterward breaks. A. guererronis pervasion prompts surface scars, decreased organic product development, and untimely organic product fall. The announced yield misfortune brought about by A. guererronis was discovered to be 34% in India. In the previous few years, a few examinations have zeroed in on the expected utilization of fundamental oil details in natural control of different bug and illnesses. The fundamental oils which get more quickly corrupted into the climate than substance compounds have been read for their activity against different bug of put away items. Late examinations have exhibited the antilarval and antifeeding, postponed grown-up rise and egg mortality, arrestant and repellant activities of fundamental oils. The current examination has been expected to utilize these characteristic subordinates as an option ecofriendly intends to control the eriophyid bugs (A. guererronis Keifer), one of the genuine bugs of coconut.              

          Research Article Pages: 1 - 8

          In Vivo Effect of Medicinal Plants on Some Serum Biochemical Parameters in Laying Hens

          Muhammad Mushtaq*, Naila Chand, Sarzamin Khan, and Umer Saddique

          Effect of medicinal plants on overall performance and serological parameters were explored in the laying birds. Forty weeks old 210-egg type birds were used as tested criteria. Birds were randomly alienated to 7 experimental groups with 3 replicates, consisted of 10 birds/replicate. Data were recorded for serology and haematology of laying birds. Serum hepatic, lipids, kidney function parameters, serum antibody titre against Newcastle disease and haematological parameters were estimated at the end of trial. Significantly lower egg yolk and serum cholesterol was recorded in group Bl-1. Medicinal plants supplementation significantly lower the triglycerides level over the control group. Significantly improve/high level of high density lipoprotein was recorded in Tg-1, Bl-1 and Ta-1, while low level of low density lipoprotein was recorded in Bl-1 and Ta-1.  Haematological parameters significantly improved in medicinal plants treated groups except haemoglobin and red blood cells counts. Hepatic biomarkers ALT and AST were recorded non-significant in all treated groups, while serum ALP and total protein was significantly altered in groups Bl-1 and Ta-0.5. Non-significant observations were recorded for nephro-protective effect of medicinal plants in laying birds. It is concluded from the results that medicinal plants possess hypolipidemic, haematological and hepato-nephro protective effects.

            Editorial Pages: 1 - 1

            Evolution of Roman Medication through the Legendary Doctors

            Nora Davis*

            Galen, the medical man of Roman medication, created a major contribution through his teachings and writings towards moving medication forward as a profession. in contrast to those of medical man, several of Galen’s works still exist as original or ancient transcripts, some even considerably older than the Venetian printing of the Hippocratic Corpus [1]. luckily, we've got rather more autobiographic data on anatomist than medical man that enables United States to follow his career mutually of Rome’s greatest physicians and provides modern classicists like Vivian Nutton concrete proof of Galen's work and existence [2]. With twenty-one volumes still living, Galen’s individual writings square measure longer in content than the whole Hippocratic Corpus. fifty three One explicit work written by anatomist that may be explored is Delaware Optimo Medico Cognoscendo, or On Examinations by that the simplest Physicians square measure Recognized, that was written around 177CE. anatomist wrote Delaware Optimo Medico Cognoscendo to supply Romans the simplest way to research doctors, however conjointly on however a doctor will become the simplest doc.54 to grasp Galen's analysis of Roman medication, we have a tendency to 1st have to be compelled to perceive Galen's history and the way it influenced the lens from that he viewed Roman medication [3].

              Research Article Pages: 1 - 5

              New RP-HPLC Method Development and Validation for Simultaneous Estimation and Forced Degradation Studies of Gallicacid and Curcumin in Solid Dosage Form

              S.K. Godasu, and S.A. Sreenivas

              Objective: A New method was established for simultaneous estimation of Gallic acid and curcumin by RP-HPLC method. Methods: Chromatographic separations were carried using Inspire (4.6 × 150 mm, 5 m) column with a mobile phase composition of 0.1% OPA buffer and Acetonitrile (30:70) have been delivered at a flow rate of 1 ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 with PDA detector 2996 at wavelength 260 nm. Results: The retention time for Gallic acid and curcumin were 2.119 and 2.730 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 500-2500 μg/ml for Gallic acid and 5-25 µg/ml for curcumin respectively. For accuracy the total recovery was found to be 100.58% and 100.54% for Gallic acid and curcumin. LOD and LOQ for gallic acid 3.05 and 10.07. LOD and LOQ for Curcumin 2.28 and 9.98. Conclusion: The results of study showed that the proposed RP?HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Gallic acid and curcumin in pharmaceutical dosage form.

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