S.K. Godasu* and S.A. Sreenivas
Objective: A New method was established for simultaneous estimation of Gallic acid and curcumin by RP-HPLC method.
Methods: Chromatographic separations were carried using Inspire (4.6 × 150 mm, 5 m) column with a mobile phase composition of 0.1% OPA buffer and Acetonitrile (30:70) have been delivered at a flow rate of 1 ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 with PDA detector 2996 at wavelength 260 nm.
Results: The retention time for Gallic acid and curcumin were 2.119 and 2.730 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 500-2500 μg/ml for Gallic acid and 5-25 µg/ml for curcumin respectively. For accuracy the total recovery was found to be 100.58% and 100.54% for Gallic acid and curcumin. LOD and LOQ for gallic acid 3.05 and 10.07. LOD and LOQ for Curcumin 2.28 and 9.98.
Conclusion: The results of study showed that the proposed RP-HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Gallic acid and curcumin in pharmaceutical dosage form.
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Journal of Pharmacognosy & Natural Products received 606 citations as per Google Scholar report
