Journal of Blood & Lymph

Chongqing Academy of Animal Sciences, Chongqing, China

Publications

• Research Article   
  Selection and Optimization of Circulating Exosomal Reference microRNAs for Quantitative Real-time PCR Studies of Porcine Body Fluids
  Author(s): Qiang Pu, Lin Wang, Guojun Kang, Changbao Liu, Changfeng Yang, Jia Luo* and Yongfu Huang*
  
  Circulating exosomal miRNAs released into all body fluids have incredible functionality and stability. Their expression is associated with multiple pathological conditions, can be used as informative biomarkers when assessing and monitoring the body’s physiopathological status. However, there is no consensus on reference miRNAs for circulating exosomal reference and abundance normalization. The present study aimed to quantify 16 potential reference miRNAs in ten porcine body fluids using qRT-PCR. Further, their stability was quantified by combining multiple gold-standard statistical tools, including BestKeeper, GeNorm, and NormFinder. The identified miRNAs were comprehensively ranked. The top-ranked miRNA was recommended as the optimal reference miRNAs for data normalization. To identify more stable genes, the body fluids were assigned into three groups based on the collection p.. Read More»
  DOI: 10.37421/ 2165-7831.2022.12.287

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