Journal of Bioprocessing & Biotechniques

Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea

Publications

• Mini Review   
  Living Things Abdominal Venous Extracellular Organelle in vitro Trackers by Applying Biotechnology Methods
  Author(s): Jaehi Kim*
  
  Despite the drawbacks of using conventional organic fluorescence probes, such as the weak fluorescent signal against the strong cellular autofluorescence background coupled with a fast-photobleaching rate, single-particle tracking (SPT) is a powerful method for exploring singlemolecule dynamics in living cells or tissues. Quantum dots (QDs), which enable tracking targets in multiple colours, have been suggested as an alternative to conventional organic fluorescence dyes. However, due to their hydrophobicity, cytotoxicity, and blinking issues, QDs are not ideal for applying SPT. The silica-coated QD-embedded silica nanoparticles (QD2) used in this study's improved SPT method have brighter fluorescence and are less toxic than single QDs. The label was kept after treatment with QD2 in 10 g/mL for 96 hours with impaired cell function, such as impaired angiogenesis,.. Read More»
  DOI: 10.37421/2155-9821.2023.13.557

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