Biosensors & Bioelectronics

Toshihisa Ohshima

Publications

• Research Article
  Efficient Direct Electron Transfer for a Highly Oriented PQQ-GDH Immobilized Electrode for Bioanode
  Author(s): Ayako Koto, Saki Taniya, Hiroaki Sakamoto, Takenori Satomura, Haruhiko Sakuraba, Toshihisa Ohshima and Shin-ichiro SuyeAyako Koto, Saki Taniya, Hiroaki Sakamoto, Takenori Satomura, Haruhiko Sakuraba, Toshihisa Ohshima and Shin-ichiro Suye
  
  A bioanode with improved enzyme orientation was developed to achieve an efficient enzyme reaction and electron transfer on an electrode surface. A highly stable PQQ-dependent glucose dehydrogenase (PQQ-GDH) isolated from a hyper-thermophilic archaeon was employed as an electron conversion element. PQQ-GDH is expected to maintain battery properties and to have a long battery life. To immobilize the enzyme onto the electrode with appropriate orientation, we introduced a His-tag to the N-terminal of PQQ-GDH by a genetic technique and utilized the affinity bond between His-tag and Cu atoms. The catalytic current density in the presence of substrate was 18.6 μA/cm2 without a mediator. The current density of the oriented electrode was approximately 90 times higher than that of the non-oriented electrode. By immobilizing the enzyme with orientation, the accessibility between the enzyme .. Read More»
  DOI: 10.4172/2155-6210.1000148

  Abstract PDF