Journal of Forensic Research

Roland Brousseau

Publications

• Research Article
  Molecular Forensic Approaches to the Taxonomic Assessment of Bacteria in a Commercial Consortia
  Author(s): Luke Masson, Thomas A. Edge, Christine Maynard, Roland Brousseau, Nathalie Fortin, Charles W. Greer and Jack T. TrevorsLuke Masson, Thomas A. Edge, Christine Maynard, Roland Brousseau, Nathalie Fortin, Charles W. Greer and Jack T. Trevors
  
  The characterization of low complexity (only a few species present) bacterial communities or commercial consortia products in terms of microbial composition can require a variety of molecular techniques for supporting forensic investigations. We examined a low complexity commercial consortium productfor water treatment application as a model for a tiered molecular approach to studying microbial communities. PCR amplification of 16S rDNA and cpn60 genes were performed on total genomic DNA extracted from the consortium. First, the PCR amplicons were cloned, sequenced and subjected to both DGGE and RFLP analysis, or they were fluorescently labeled and hybridized to a dual backbone taxonomic DNA microarray. Secondly, total genomic DNA from the commercial consortium was subjected to quantitative PCR to determine the concentration of the different components. T.. Read More»
  DOI: 10.4172/2157-7145.S2-002

  Abstract PDF