Biosensors & Bioelectronics

ISSN: 2155-6210

Open Access

Kalabova H

Faculty of Biomedical Engineering,
Czech Republic

  • Research Article
    Mathematical Model for Laboratory System of Bioluminescent Whole-Cell Biosensor with Optical Element
    Author(s): Kalabova H, Pospisilova M, Jirina M and Kuncova GKalabova H, Pospisilova M, Jirina M and Kuncova G

    The fiber-optic biosensor with encapsuled bioreporters use a special optical element (OE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector for on-line measurement in remote localities. The active layer of biosensor contents bioluminescent bioreporters – genetic modified cells, which are sensitive to its surrounding environment-immobilized in silica matrix on the end of OE. Bioreporters are able to react by emission of visible light (≈500 nm) – bioluminiscence reaction (BL) – in presence of specific analyte. The genetic modified bacterial stain Pseudomonas putida TVA8 was chosen as biorecognition part of biosensor producing BL in the presence of benzene, toluene, ethylbenzene and xylens (BTEX). Very low level of BL signal was detected by high-sensitive photon-counter. The sensitivity of b.. Read More»
    DOI: 10.4172/2155-6210.1000250

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