Using transcriptome sequencing technology to screen key genes related to HIV-1 unsusceptibility from HEPS subjects in China

4^th International Conference on HIV/AIDS, STDs and STIs

October 03-05, 2016 Orlando, Florida, USA

Hui Chen, Jiegang Huang, Junjun Jiang, Bingyu Liang, Jie Liu and Li Ye

Guangxi Medical University, China

Posters & Accepted Abstracts: J AIDS Clin Res

Abstract :

Understanding of unsusceptibility mechanisms of HIV-1 highly exposed and persistently seronegative (HEPS) individuals may provide new treatment strategies or vaccine targets. Although a mutation known as CCR5Ã?Â?32 in the CCR5 gene can protect certain individuals against HIV infection, the CCR5Ã?Â?32 genotype is rarely found in Chinese population, suggesting Chinese HEPS persons have other unsusceptibility mechanisms. We used transcriptome sequencing technology to screen key genes related to HIV- 1 unsusceptibility from HEPS subjects in China. HEPS subjects were strictly selected according to the HEPS standards. Age, gender and ethnic-matched healthy individuals were enrolled as control. Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood samples and total cellular mRNA was then extracted for high-throughput transcriptome sequencing (Hi-Seq 2000). Totally, there were 1906 differentially expressed genes between one HEPS sample (GH01) and one control sample (C01), with 1139 up-regulated genes and 767 down-regulated genes in sample GH01. Among them, 15 genes have been reported to be associated with HIV-1 unsusceptibility and 30 genes have been reported to be related to HIV-1 infection/replication, however, no reports show their relationship to HIV-1 unsusceptibility. GO function enrichment and KEGG pathway analyses show seven significantly differential pathways (Q value<0.05) between two samples. Among them, two pathways, including natural killer cell mediated cytotoxicity pathway and antigen processing and presentation pathway, have been reported to be closely associated with HIV-1 infection. The above data lay a solid foundation for the future research on specific mechanisms responsible for HIV-1 unsusceptibility in HEPS subjects in China.

Biography :

Email: chenhui680804@163.com