Ultra-structural characterization of renal interstitial fibrosis in cats with chronic disease

Journal of Nephrology & Therapeutics

ISSN: 2161-0959

Open Access

Ultra-structural characterization of renal interstitial fibrosis in cats with chronic disease

8th World Nephrology Conference

August 15-16, 2016 Sao Paulo, Brazil

Janaina Serra Azul Evangelista

Federal University of Ceara, Brazil

Posters & Accepted Abstracts: J Nephrol Ther

Abstract :

Kidney Interstitial fibrosis leads to a progressive loss of nephrons and has been clearly correlated with decreased renal function and consequent chronic disease. This study demonstrated the deposition and arrangement of collagen fibers in cat kidneys with chronic disease. We studied 10 cats with chronic kidney disease. After death, kidneys were sectioned and fixed in 4% paraformaldehyde and 2.5% glutaraldehyde solution. Then, samples were washed in 0.1M phosphate buffer and transferred to osmium 2% solution. After that, the process of dehydration was started and 2% uranyl acetate was added. At this time, samples were washed with propylene oxide solution and embedded in Araldite resin at 70├?┬░C for 3 days. The resin blocks were cut in semithin sections in ultra-microtome and further selection areas to ultrathin sections to be analyzed in transmission electron microscopy. This work was approved by the Ethics Committee for Animal use. The thin sections showed strong deposition of interstitial collagen, periglomerular and peri-vascular inflammatory cells and fibroblasts. In the transmission electron microscopy, the fragments had arranged collagen fibers around the glomeruli and tubules with streaks dense electron. We observed areas with many fibers arranged longitudinally forming beams. We concluded that the analysis of morphological changes in cat kidneys with chronic disease, through studies of the cells involved the occurrence of fibrosis and tubular damage are extremely important and necessary for greater understanding of the evolution and progression of this disease in cats.

Biography :


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