Trisulfate disaccharide decreases calcium overload and protects liver injury secondary to liver ischemia/reperfusion

9^th International Conference on Nephrology: Kidney & Therapeutics

September 29-30, 2016 Orlando, USA

Enio Rodrigues Vasques, Jose Eduardo Monteiro Cunha, Ana Maria Mendon�§a Coelho, Sandra N Sampietre, Rosely Antunes Patzina, Emilio Elias Abdo, Helena B Nader, Ivarne LS Tersariol, Marcelo Andrade de Lima, Carlos MG Godoy, Rodrigues, Tiago, Eleazar Chaib and Luiz A Carneiro Dâ��Albuquerque

Sao Paulo University, Brazi
Federal University of Sao Paulo (UNIFESP), Brazil
Federal University of ABC, Brazil

Posters & Accepted Abstracts: J Nephrol Ther

Abstract :

Background: Ischemia and reperfusion (I/R) causes tissue damage and intracellular calcium levels are a factor of cell death. Sodium calcium exchanger (NCX) regulates calcium extrusion and trisulfate disaccharide (TD) acts on NCX decreasing intracellular calcium through the inhibition of the exchange inhibitory peptide (XIP). Objectives: The aims of this research are to evaluate the TD effects in liver injury secondary to I/R in animals and in vitro action on cytosolic calcium of hepatocytes cultures under calcium overload. Methods: In this study, Wistar rats submitted to partial liver ischemia were divided in to groups: Control: (n=10), surgical manipulation with no liver ischemia; Saline: (n=15), rats receiving IV saline before reperfusion; and TD: (n=15), rats receiving IV TD before reperfusion. Four hours after reperfusion, serum levels of AST, ALT, TNF-�±, IL-6, and IL-10 were measured. Liver tissue samples were collected for mitochondrial function and malondialdehyde (MDA) content. Pulmonary vascular permeability and histologic parameters of liver were determined. TD effect on cytosolic calcium was evaluated in BRL3A hepatic rat cell cultures stimulated by thapsigargin pre and post treatment with TD. Results: AST, ALT, cytokines, liver MDA, mitochondrial dysfunction and hepatic histologic injury scores were less in TD group when compared to Saline group (p<0.05) with no differences in pulmonary vascular permeability. In culture cells, TD diminished the intracellular calcium raise and prevented the calcium increase pre and after treatment with thapsigargin, respectively. Conclusion: TD decreases liver cell damage, preserves mitochondrial function and increases hepatic tolerance to I/R injury by calcium extrusion in Ca2+ overload situations.

Biography :

Email: evasques@globo.com