GET THE APP

Thin layer chromatography methods in the characterization and stability analyses of the aqueous and dichloromethane extracts of Lagerstroemia speciosa L. (fam. Lythraceae) leaves
..

Medicinal Chemistry

ISSN: 2161-0444

Open Access

Thin layer chromatography methods in the characterization and stability analyses of the aqueous and dichloromethane extracts of Lagerstroemia speciosa L. (fam. Lythraceae) leaves


2nd International Conference on Medicinal Chemistry & Computer Aided Drug Designing

October 15-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

J. L. Gonzaga, J. S. Bonifacio, J. R. Dumbrique, J. O. Gutierrez, C. R. Jimenez, C. S. Mendoza, P. B. Polido, M. G. Rasalan, I. R. Sampang, M. S. Siocson, C. M. So, V. D. Solis and M. J. Taguinod

Accepted Abstracts: Med chem

Abstract :

Lagerstroemia speciosa L. (Fam. Lythraceae), or Banaba, is traditionally used for diarrhea, abdominal pain, and diabetes. Identification of Banaba in the Philippine pharmacopeia employs one-dimensional thin layer chromatography (TLC) and shows three separated components from the extracts of Banaba. In this study, the aqueous and dichloromethane (DCM) fractions of the methanolic extract of Banaba were subjected to two-dimensional TLC using acetone: chloroform and n-hexane: ethyl acetate: glacial acetic acid solvent systems. Visualization was done using iodine vapors, short- and long-wave UV light (254 nm and 365 nm), and vanillin-sulfuric-phosphoric acid spray reagent. Efficient separation was more clearly observed in the DCM fraction chromatogram. The aqueous fraction, thereby, was subjected to reverse phase thin layer chromatography (RPTLC) wherein a 1% acetic acid: acetonitrile (70:30) solvent system yielded the best resolution. Stability analyses using TLC in both aqueous and DCM fractions of the extracts of leaf samples pre-exposed to UV light for 12 and 24 hours and controlled temperature of 40C and 60C for three hours showed that only the increase in temperature caused a significant change in the number of spots separated and detected. Two-dimensional RPTLC analysis or one-dimensional RPTLC analysis employing gradient elution may be applied to the aqueous fraction to investigate if separation can further be optimized. Keywords: Banaba, Two-dimensional thin layer chromatography, Reverse phase TLC, Stability analysis

Google Scholar citation report
Citations: 6627

Medicinal Chemistry received 6627 citations as per Google Scholar report

Medicinal Chemistry peer review process verified at publons

Indexed In

 
arrow_upward arrow_upward