The protective effects of Purα on rat hippocampus DNA damage induced by Epilepsy

Neurological Disorders

ISSN: 2329-6895

Open Access

The protective effects of Purα on rat hippocampus DNA damage induced by Epilepsy

International Conference on Epilepsy & Treatment

September 21-22, 2015 Baltimore, USA

Jianqi Cui, Qiang Liu, Juan Chai, Zheng Yang, Zuyao Song, Zhengquan He, Jianguo Niu, Feng Wang and Tao Sun

Ningxia Medical University, China

Posters-Accepted Abstracts: J Neurol Disord

Abstract :

Objective: To investigate the protective effects of Pur?± protein on rat hippocampus DNA damage induced by epilepsy and the effects of Pur?± protein on DNA damage and repair. Methods: The lentivirus overexpression and SiRNA constructs of Pur?± were packaged in vitro and the high tittered virion was injected into rat hippocampus guided by stereotaxic apparatus. The experimental animals were divided into 4 groups: Control, overexpression, SiRNA and empty vector groups. The level of Pur?± expression and knock down were checked in the 14 days after the virion injection with fluorescent slides and western blotting to confirm that virus has already infected the hippocampus tissues. The Pilocarpine was used to induce epilepsy by abdominal injection. The experimental animals were executed 1 hour after the epileptic onset and the hippocampus samples were collected for immunohistochemical staining and western blotting assay to examine the pertinent protein expression to investigate the protective effects of Pur?± on DNA damage and repair. Results: Pilocarpine can induce epileptic onset, immunohistochemistry demonstrated that ?³H2AX, a landmark protein for DNA damage, has higher expression in CA1 region of rat hippocampus. The damage became aggravated when Pur?± protein has been knocked down but in Pur?± overexpression group, the damage became alleviated obviously. The results of western blotting illustrated that the proteins associated with DNA damage such as Parp-1, Ku80, XRCC4 has higher expression level when Pur?± was knocked down but on the other hand, these proteins have lower expression when Pur?± was overexpressed. Conclusion: The DNA damage can occur in the early stage of epilepsy onset, Pur?± protein can protect the DNA damage caused by epilepsy and also participated in the repair process of DNA damage.

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