The design of a large-scale filamentous spore preparing device

4^th International Conference on Bioprocess and Bio Therapeutics

October 20-21, 2016 Houston, USA

Xiaoran Zhang and Wenxia Jiang

Tianjin Institute of Industrial Biotechnology-CAS, China

Posters & Accepted Abstracts: J Bioproces Biotechniq

Abstract :

In fermentation industry, the mechanization level of inocula preparing procedure used to be the lowest in fermentation processes. Particularly, amplification culturing of filamentous spores are generally considered to be difficult, although they are producers of numerous compounds, such as antibiotics, organic acids, enzymes and biological pesticides. Thus, it is necessary to develop a new large-scale filamentous spore preparing device, which could decrease the labor intensity, elevate the mechanization level and improve the reliability of the inocula. Spores are usually amplification cultured by surface cultivation. For example, Aspergillus niger in citric acid plants in China are commonly pre-cultured in a medium composed of bran in numerous Erlenmeyer flasks, and a large amount of Erlenmeyer flasks are needed to prepare the mouldy bran. To decrease the labor intensity, elevate the mechanization degree and improve the reliability of the inocula, a new large-scale filamentous spore preparing device was designed, which contains two subsystems: The solid-state culturing subsystem and spore-separating subsystem. Solid-state culturing subsystem employed a flexible agitating mechanism which could agitate the media across a thick layer of supporting balls without touching the media and filamentous spore directly. Spore-separating subsystem employed a cyclone separator and a spore-collecting bottle. Vacuum generator could provide additional differential pressure, and pneumatic vibrator could help to collect the spores adhered to the inside wall.

Biography :

Email: zhang_xr@tib.cas.cn