Systems approaches for understanding the HIV-host functional interface

Nevan J. Krogan

: J Antivir Antiretrovir

Abstract :

Dr. Krogan is an Associate Professor in the Department of Cellular and Molecular Pharmacology at the University of California-San Francisco and is an expert in the fields of functional genomics and systems biology. He was born and raised in Regina, Saskatchewan, Canada and obtained his undergraduate degree from the University of Regina. As a graduate student at the University of Toronto, Dr. Krogan led a project that systematically identified protein complexes in the model organism, Saccharomyces cerevisiae , through an affinity tagging-purification/mass spectrometry strategy. This work led to the characterization of 547 complexes, comprising over 4000 proteins, and represents the most comprehensive protein-protein interaction map to date in any organism. To complement this physical interaction data, Dr. Krogan developed an approach, termed E-MAP (or epistatic miniarray profile), which allows for high-throughput generation and quantitative analysis of genetic interaction data. Dr. Krogan?s lab at UCSF focuses on applying these global proteomic and genomic approaches to formulate hypotheses about various biological processes, including transcriptional regulation, DNA repair/ replication and RNA processing. He is now developing and applying methodologies to create genetic and physical interactions between pathogenic organisms, including HIV and TB, and their hosts, which is providing insight into the human pathways and complexes that are being hijacked during the course of infection