Rapid analysis of hemoglobin variants in beta-thalassemia by HPLC in northern India

5^th International Conference and Exhibition on Metabolomics

May 16-18, 2016 Osaka, Japan

Yashwant Kumar Rao

GSVM Medical College, India

Posters & Accepted Abstracts: Metabolomics

Abstract :

Introduction: Beta-thalassemia is the most common and hereditary blood disorder worldwide, most of which are characterized by base substitution or small deletion or insertion of one or two nucleotides in the globin gene. Today due to the mixture of gene pool, this disorder is now not confined to any particular ethical group/races but each group represents its own sets of mutations. High Performance Liquid Chromatography forms a rapid, sensitive and precise method for detecting abnormal hemoglobin fractions. About 55 cases of Beta-thalassemia have been studied for various hemoglobin variants from Kanpur and adjoining areas. Material & Method: The study was performed on Agilent 1220 Infinity LC (Agilent Technologies) a High Performance Liquid Chromatography using EZChrom Elite for Beta-thalassemia. Result: Abnormal hemoglobin variants were analyzed for 55 cases of Beta-thalassemia on High Performance Liquid Chromatography. There were about 18 cases of Beta-thalassemia major and 37 cases of Beta-thalassemia carriers. The frequency observed in our study was HbA1c (0.14), HbF (0.7), HbE (0.45), HbD (0.34), HbS (0.45), and HbA2 (0.52). Conclusion: Automated High Performance Liquid Chromatography is an appropriate approach for the screening and presumptive identification of patients as well as carriers of Beta-thalassemia prior to DNA studies for definitive diagnosis.

Biography :

Email: ykraoneo@yahoo.co.in