InduShekhar Thakur and Randhir Kumar Bharti
Carbon dioxide is one of the Green House Gases may be mitigated by sequestration of microorganismsand production of biomass and biofuels. Microorganisms isolated from the sediment and water of marble rock was enriched in minimal salt media with different concentrations of sodium bicarbonate, CO 2 and light source. Microorganisms were identified aschemolithotrophic bacteria, Serretiamarcescens ;cynobacteria, Lyngbya spp., by 16S RNA sequencing, and microalgae as Chlorella sp. Proteomics approaches evaluated by 2D gel electrophoresis and MALDI-TOF/MF and genomicsby amplification and analysis of carboxylating genes indicated presence of carbonic anhydrase, ribulose-1, 5- bisphosphate carboxylase/ oxygenase,acetyl-CoA carboxylase,propionyl-CoA carboxylase, succinyl-CoA synthetase, pyruvate synthase, 3-hydroxybutyryle dehydrogenase, succinate dehydrogenase, pyruvate dehydrogenase, malate/L-lactate dehydrogenase and citrate synthaseenzymes related to sequestration of carbon dioxide, and acyl CoA dehydrogenase, fatty acid desaturase type 1,lipoic acid synthatase, enoyl CoAhydratase, branched chain alpha?keto acid dehydrogenase, holo-acyl carrier protein synthase and tartrate dehydrogenase for fatty acid metabolism. GC-MS results indicated formation of major organic compounds,dodecane 1-fluoro by Chlorella sp.,heptylcyclohexane by Lyngbya spp. and cyclododecane methanol by Serretiamarcescens. Chlorella sp. applied for sequestration of CO 2 at different concentrations (0.05%, 5%, 10% and 15%) produced lipid 15.0, 25.0, 26.0 and 28.3mg/L/d respectively and produced biodiesel (7.02 g/ L) with calorific value 29.8kJ/g suggested possible scale up the method for low carbon society.
InduShekhar Thakur graduated and obtainedPh.D. from the Jawaharlal Nehru University, New Delhi, India. He is Professor in the School of Environmental Sciences, JNU, New Delhi. He published more than 75 research papers in reputed journals and. two books. He has patents and technology related to bioremediation, bioconversion and detoxification of organic and inorganic compounds in the environment.